Manuals - Bulletins - Drawings
802D Permeabilized Fiber Test Apparatus
>> Click to enlarge picture
(Pictured with 312C Length Controller and 403A Force Transducer)
Features
- 8-well, temperature controlled bath plate
- Automatic indexing from any bath to any other at the push of a button
- Temperature controlled 0-40C
- XYZ micrometer translators included
- Bath volume of either 120 µl or 160 µl
- Bath 1 designed for fiber attachment and features right angle prisms for visualization of the side of the fiber
- Microprocessor-based motion controller and TEC controller
- Corrosion resistant materials used throughout
The 802D permeabilized fiber test apparatus was designed to enable physiology researchers to easily test permeabilized fibers with an ASI model 315C or 322C high-speed length controller and an ASI series 400A force transducer.
The apparatus consists of a temperature controlled 8-well bath plate, indexing system, microprocessor based controller, and XYZ micrometer translation stages that include mounts for a lever system and force transducer. All parts are manufactured from corrosion resistant materials (anodized aluminum, stainless steel and Delrin).
Simple Push Button Control
The 802D works by moving the bath plate relative to the fiber. The fiber is clamped between a force transducer and a high-speed length controller. In operation the researcher selects the desired bath by either clicking a button on the 802D PC Software, or by running a protocol from our ASI600A software. When a new bath is selected the bath moves downwards and then moves to the desired bath. When it arrives at the new position the plate automatically rises to the operating position. The on-screen software includes a digital display that shows the current bath position. Bath-to-bath index time is approximately 1.2 seconds. This includes the time required to lower the bath, move to the new position and then raise it. Position sensors for each bath ensure positioning accuracy to better than +/-0.05mm.
Eight Well Bath Plate
The bath plate is manufactured from aluminum with an inert coating of Tufram that provides corrosion protection. The plate has eight baths machined in its upper surface. This number allows for test protocols involving multiple solutions without the need to empty and re-fill baths. All baths have a glass bottom to allow for microscope observation of the fiber or laser-based sarcomere length measurement. Bath 1 is larger than the rest to allow easy fiber attachment to the force transducer and length controller. Bath 1 also includes right angle prisms on both sides of the bath to allow fiber dimensions to be measured from the side as well as from the top.
Includes Mounts and XYZ Translation Stages
Both the high-speed length controller and the force transducer are mounted on XYZ micrometer translation stages to allow them to be positioned relative to the bath plate.
Software Programmable Motion Sequencer
The 802D Bath Controller features a software programmable motion control sequencer. This sequencer allows the researcher to automate the movement of the bath plate.
Temperature of the test plate can be controlled from 4 to 40C within +/- 0.1C using the supplied ASI model 825A thermoelectric controller. The 825A also contains a low-noise thermocouple thermometer with both a digital readout and a voltage output. An ASI model 826A Water Circulator is also included.
Specifications
Bath Plate
- No. of Baths: 8
- Bath Plate Material: 6061-T6 Aluminum
- Right-Angle Prisms: 3x3x6mm, aluminized hypotenuse with incolnel overcoat and black paint overcoat
- Bath Dimensions:
- Large Volume Bath Plate:
- Bath 1: 14mm (0.551”) L x 5mm (0.197”) W x 3 (0.118”) D
- Baths 2 – 8: 14mm (0.551”) L x 4mm (0.157”) W x 3mm (0.118”) D
- Small Volume Bath Plate:
- Bath 1: 14mm (0.551”) L x 5mm (0.197”) W x 3 (0.118”) D
- Baths 2 – 8: 14mm (0.551”) L x 3mm (0.118”) W x 3mm (0.118”) D
- Bath Volume:
- Large Volume Bath Plate:
- Bath 1: 198 µl
- Baths 2–8: 158 µl
- Small Volume Bath Plate:
- Bath 1: 198 µl
- Baths 2–8: 120 µl
- Centerline Distance X-axis:
- Bath 1 – 2: 8 mm (0.315”)
- Baths 2 – 8: 6 mm (0.236”)
- Angular Movement, Z-axis: 3.0°
- Excursion, Z-axis:
- Max. (at FT end of Bath ): 4.72 mm (0.186”)Min. (at lever end of Bath): 3.35 mm (0.132”)
- Indexing Time:
- Bath 1 – Bath 2: 1.2 seconds
- Adjacent Baths in range 2-8: 1.1 seconds
- Bath 1 – Bath 8: 2.5 seconds
Thermoelectric Coolers
- No. of TECs: 2
- Power: 33 W
- Voltage: 15 V
- Temperature Sensor: AD590
Thermocouple Microprobe
- Model: Physitemp IT-18
- Temp Range: -273 to +150C
- At tip: -492 to+300F
- Time Constant: 0.1 s
- Sensor: Type T Thermocouple Copper-Constantan
- Sheath: 0.025" (0.064mm) diameter. Teflon with sealed tip.
Stepper Motors
- No. of Motors: 2
- Voltage: 12 V
- Movement: 25mm (0.001”)/step
General
- Warm Up Time: 30 seconds to rated accuracy
- Power Requirements: 100–240VAC±10%, 50/60Hz, 2 amps max